This study was designed to evaluate the effects of culture-medium temperatures on the growth of known quantities of in vitro inoculum of Neisseria gonorrhoeae. Growth of N gonorrhoeae was significantly greater on room-temperature culture medium than on refrigerated culture medium with inocula of 10(2) and 10(3) colony-forming units. There was no difference between growth patterns on room-temperature and those on refrigerated media when inocula were 10(5) and 10(6) colony-forming units.