Abstract
Context: The effects of maternal hypoglycemia on birth weight, placental weight, and placental ratio are unclear. A reliable indicator for increased risk of low birth weight or associated low placental weight could prove invaluable in caring for newborns who are small for gestational age.
Objective: To retrospectively review 2 years of hospital obstetric records for evidence of an association between maternal hypoglycemia (< fifth percentile at 24-week 1-hour glucose challenge test) and birth weight, placental weight, or placental ratio.
Methods: Medical center records were reviewed for women who delivered a term newborn between July 1, 2005, and July 31, 2007. Women included in the study were younger than 35 years and had completed a 1-hour glucose challenge test during pregnancy. Excluded were women with comorbid conditions that may be associated with abnormal birth weight and women with serum glucose levels greater than 135 mg/dL.
Results: Newborns of women with hypoglycemia weighed, on average, significantly less than newborns of women with normal blood glucose levels (t test P=.011). Relative risk was 5.81 (95% confidence interval, 1.25-27.03). Placentas of women with hypoglycemia were also lighter than those of the women in the control group, but the difference was not significant (t test P=.1089). Differences in placental ratios between the 2 groups were not statistically significant (P=.8171).
Conclusion: Lower serum glucose levels during pregnancy might be a causative factor, rather than merely a risk factor, for lower birth weights. (ClinicalTrials.gov number NCT00614094)
During pregnancy, rising estrogen levels stimulate insulin production by the pancreas beta cells, causing changes in glucose metabolism. As the fetus draws on maternal fat stores, ketosis can develop and lead to hypoglycemia, particularly during periods of fasting.
1
Several studies have addressed the possible effects of maternal hypoglycemia on birth weight, placental weight, or placental ratio (ratio of placental weight to birth weight). The most common finding is that maternal hypoglycemia is associated with an increased risk of intrauterine growth retardation (IUGR) or a small for gestational age (SGA) newborn.
2-8 How-ever, some reports have contradicted that association.
9-11 Although few studies have examined a potential association between maternal hypoglycemia and altered placental weight or placental ratio,
12 reports of some studies have indicated an association between SGA newborns and increased placental weight or placental ratio.
13-14 Other studies, however, have found just the opposite.
15,16
Confounding the interpretation of data on the effects of maternal hypoglycemia are variability in values used to define hypoglycemia, timing of serum glucose measurement (ie, whether it was measured at 1 hour or 3 hours after administration of oral glucose solution), and consideration of comorbid factors such as smoking or drug abuse, which in themselves would help to explain SGA newborns.
2,5
In short, the effects of maternal hypoglycemia on birth weight, placental weight, and placental ratio are still unclear. However, a reliable indicator for increased risk of low birth weight or associated low placental weight could prove invaluable in light of outcomes for SGA newborns. In addition to being at increased risk for hypoglycemia, sepsis, seizure, stillbirth, respiratory distress, and meconium aspiration, SGA newborns are at risk for long-term outcomes such as poor school performance, hyperactivity, hypertension, and cardiovascular disease.
17,18
The purpose of the present retrospective study was to assess whether maternal hypoglycemia is a contributing cause of decreased birth weight, altered placental weight, or altered placental ratio.
All statistical analyses were conducted with the use of PC SAS statistical software (Version 9.1; SAS Institute Inc, Cary, North Carolina). Both the relationship of birth weight to maternal hypoglycemia and the relationship of placental weight to maternal hypoglycemia were investigated with 2 different statistical procedures: a
t test and a contingency table. The placental ratio was analyzed by a
t test but not with a Fisher exact test. The contingency table and Fisher exact test required categorization of all variables. Although it is known that a normal placental ratio is 1:6, or 0.167,
19 reference ranges are not yet well established, so each woman's placental ratio could not be objectively labeled as “normal” or “not normal.”
For t test analysis, birth weight and placental weight were defined according to continuous and meaningful numeric values rather than categories such as “low,” “normal,” or “high.” These values were used to determine whether statistically significant differences existed between the study group (ie, women with hypoglycemia) and the control group (ie, women with normal blood glucose levels) with regard to the mean birth weight, the mean placental weight, and the mean placental ratio.
Categories for birth weight included “SGA” and “not SGA.” For analysis, SGA was defined as birth weight below the 10th percentile for gestational age.
17,20 In the majority of cases, gestational age was confirmed with first trimester ultrasonography. Most women included in this study underwent ultrasonography before 20 weeks gestation, making the recorded gestational ages of the newborns reliable and accurate. To compare the birth weight category (ie, “SGA” vs “not SGA”) with the maternal hypoglycemia category (ie, study group vs control group), a contingency table was constructed. Because the sample number for births was relatively small, a Fisher exact test was used to assess the relationship.
The weight of small placentas was also categorized as less than the 10th percentile for gestational age.
21 Like the birth weight analysis, the placental weight category (ie, “low” vs “normal”) and the maternal hypoglycemia category (ie, study group vs control group) were compared using a contingency table, and a Fisher exact test was used to assess the relationship.
As was previously explained, no contingency table was created for placental ratio.
The present data agree with data from other studies that have found a relationship between low birth weight and maternal hypoglycemia.
2-8 Our study differs from previous studies, however, in that women in the present study who had other risk factors for altered birth weights were excluded from the sample. Because of this exclusion, our results suggest a cause-and-effect relationship rather than just an association between maternal hypoglycemia and low birth weight. In addition, the
t test
P value for association between hypoglycemia and lower placental weight was fairly close to statistical significance (.1), even with only 157 women included in the study. The results seem to suggest that maternal hypoglycemia is, in fact, associated with slightly lighter placentas on average, but this difference would only reach statistical significance with larger sample numbers. The
P value from the Fisher exact test for maternal hypoglycemia and lower placental weight was further from statistical significance (.24). In light of the other results, the
P value for placental ratio was expectedly far from statistical significance because the birth weights and the placental weights in the study group trended in the same direction away from those in the control group.
One positive finding in the present study is confirmation of results obtained with 2 different statistical tests: the t test and the Fisher exact test on the contingency table. Another strength, as previously mentioned, is the study's exclusion of women who had conditions that are known to affect birth weight or placental weight.
However, because we screened for a number of comorbid conditions, this study had a weakness of a relatively small sample size. If we had reported the serum glucose results of every person who took the glucose challenge test, about 5% of the results would have been in the fifth percentile or below. Instead, about 38% of our usable charts had values less than or equal to the fifth percentile. Excluding women with high serum glucose levels from the pool of participants would cause the remaining women with low and normal serum glucose levels to occupy a larger percentage of the total of women who had their blood glucose evaluated.
The modest number of patients delivering newborns at Oklahoma State University Medical Center overall also explains why a 2-year study would yield 157 usable charts. The scope of this project reflects the fact that our clinic serves patients in an urban population, many of whom have risk factors that excluded them from the study. However, it should be noted that for the t tests performed, the sample sizes were powerful enough to detect a departure of 1 standard deviation from the baseline with 99% likelihood.
Although our birth weight results agreed with results in the majority of former reports we reviewed on the subject,
2-8 these previous studies did not typically control for other factors that are known to be associated with SGA newborns. Some of the former studies based their findings of hypoglycemia on serum glucose measurements taken 3 hours after oral glucose intake,
2-5,8 rather than 1 hour after intake, as we did. Other studies found no statistically significant association between hypoglycemia and SGA newborns,
9-11 and 2 of those reports did control for such SGA-associated conditions as preterm birth, hypertension, smoking, and drug abuse.
9-11 The present data suggest that maternal hypoglycemia could be a causative agent, lending support to an unsubstantiated previous claim.
2
It should be noted, however, that some unknown third factor could also be the cause of both SGA newborns and hypoglycemia. In addition, SGA newborns and hypoglycemia could be merely coincidental. For these reasons, causality cannot be established with certainty.
Some past studies have noted a relationship between SGA newborns or IUGR and increased placental weight or placental ratio,
13,14 which the authors presumed was due to placental attempts to compensate for low nutrient uptake by the fetus. However, one study
15 reported just the opposite: SGA newborns had placentas that were 45% smaller than the control patients. Nevertheless, this particular relationship was not the focus of our study, and a possible association with hypoglycemia was not considered or discussed in any of those articles.
3
Studies that actually examine placental weight in relation to maternal hypoglycemia, rather than only SGA or IUGR, are scant. Aldoretta et al
12 found that hypoglycemic sheep mothers had lighter placentas than control sheep. The
t test
P value of our work appears to trend toward agreement with the
P value obtained by Aldoretta et al. The accepted value for a normal placental ratio is about 1:6, or 0.167.
19 The ratios of both the mothers with hypoglycemia and the mothers with normal blood glucose levels in the present study were very close to that value at 0.17. Consequently, due to low sample size—even though the
P value of the
t test was close to statistical significance—it seems unlikely that repeated tests would reveal a lower value, particularly in light of the fact that the
t test result was not corroborated by a near statistically significant
P value in the Fisher exact test.
Just as hyperglycemia is associated with larger birth weight newborns, hypoglycemia might hinder fat-storing or growth-promoting aspects of the fetus' environment, which could result in smaller birth weight newborns.
5,6 Further, the same mechanism that results in an SGA infant might also be responsible for a smaller placenta. A good follow-up study might have larger numbers of patients and a more stringent definition of hypoglycemia. The present study included too few women whose serum glucose levels were below 60 to make the study group any more exclusive than less than or equal to the fifth percentile. This less rigorous definition of hypoglycemia corresponded to the protocol of some previous research
2,9,10 and strengthens our conclusions about the relationship between maternal hypoglycemia and low birth weight.