Abstract
Celiac disease is a gastrointestinal disorder characterized by inflammation, leading to injury to the mucosal lining of the small intestine. The inflammation occurs when gliadin, a protein found in such gluten-containing foods as wheat, rye, and barley, is ingested by genetically susceptible individuals. The mucosal damage and subsequent malabsorption of nutrients leads to various complications. Researchers estimate that more than 2 million people in the United States have celiac disease—a prevalence that is greater than was previously believed. Approximately 60,000 Americans are diagnosed annually with celiac disease. Until recently, diagnosis has been complicated by the fact that the indicators of celiac disease are nonspecific. However, because of the development of new, easy-to-administer serology tests, diagnosis has become much less complicated. After conducting a review of the literature, the authors recommend a serologic testing sequence for diagnosis of celiac disease and urge that adults and children with an assortment of symptoms be tested for this disease. Common signs and symptoms of celiac disease include anemia, arthralgia, fatigue, infertility, neuropathy, and weight loss, in addition to such gastrointestinal symptomatology as abdominal pain, anorexia, bloating, constipation, and diarrhea. The only treatment for patients with celiac disease remains a gluten-free diet.
In the not too distant past, celiac disease was rarely included in differential diagnoses. Instead, this gastrointestinal disorder was usually considered just another uncommon medical condition. However, with the advent of new diagnostic technologies, researchers have discovered that the prevalence of celiac disease in the United States ranges as high as 4.54% in close relatives of individuals with the disease, similar to the prevalence reported in Europe.
1 More than 2 million people in the United States—or approximately 1 in 140 individuals—are likely to have celiac disease.
1,2 Previous estimates had placed the celiac disease-prevalence rate in the United States at 1 in 3000 people.
2 Approximately 60,000 Americans are diagnosed annually with celiac disease.
2
Thousands of patients in the United States suffer needlessly from celiac disease. Yet, these patients are not without symptoms—though the symptoms may be subtle. For this reason, physicians need to consider celiac disease in their differential diagnoses for many of their patients. The goals of the present article are to increase physician awareness of celiac disease and to outline a strategy for diagnosis and treatment to benefit the greatest number of patients.
The United States National Library of Medicine's MEDLINE database was searched for English-language articles on celiac disease published between January 1996 and December 2003. Key terms used in the search were celiac disease, gluten-sensitive enteropathy, serological testing, and treatments. After pertinent articles were identified, a group of healthcare providers who had an interest in the identification of patients with celiac disease was organized. This group, which consisted of the authors, met on several occasions to formulate practitioner guidelines for the identification of patients with this disorder. These guidelines were based on a review of 60 articles.
The assortment of symptoms experienced by individuals with celiac disease can describe many patients who enter the office of the family physician. Because of these variable and sometimes subtle manifestations, celiac disease is underdiagnosed in the adult population in the United States.
1–3
The best approach to diagnosing celiac disease appears to be a systematic process of case identification, targeting those patients with signs, symptoms, or conditions associated with celiac disease.
1 When such patients are identified, appropriate serologic testing can begin (
Figure 3).
To confirm a physician's suspicion that a patient has celiac disease, we recommend a multistep, cost-effective approach to serologic testing (
Figure 4). The first step is to conduct tests for both immunoglobulin A-tissue transglutaminase (IgA-tTG) and total serum IgA. The test for total serum IgA is suggested because there is a high prevalence of IgA deficiency in patients with celiac disease.
16
If results of both of these tests are negative, celiac disease is unlikely. However, if the tests are negative but a high clinical suspicion for the disease remains, we suggest testing for IgA antibody to endomysial antibody (IgA-EMA). If results of either the IgA-tTG or the IGA-EMA test are positive, the patient should be referred to a gastroenterologist for a definitive diagnosis, which may include a duodenal biopsy.
If the IgA-tTG test results are negative, but total serum IgA levels are low—suggesting an IgA deficiency—we recommend testing for immunoglobulin G (IgG). If results of this test are negative, celiac disease is unlikely. A positive finding in the IgG test should be followed by a tissue biopsy of the small intestine.
Many children under 2 years of age lack EMA and tTG antibodies, resulting in false-negative testing.
2,17 Because of this possibility, tests for IgA antigliadin antibodies (IgA-AGA) and IgG antigliadin antibodies (IgG-AGA) should be added to the diagnostic protocol for these individuals. These antibodies are the most useful serologic markers in symptomatic children younger than age 2.
2 However there are limitations to using the IgA-AGA and IgG-AGA tests for celiac disease because these antibodies may also be found in healthy individuals and in individuals with other inflammatory bowel conditions.
2 Thus, serologic testing for very young children—particularly those under the age of 5—is unreliable and requires further study.
3
All serology tests should be performed
before eliminating gluten from the diet. This is because the results of any later serologic analyses would be altered by such a diet.
18 The use of immunosuppressants would also alter the results of serologic analyses, so these medications, too, should be avoided until after testing.
18 If a gluten-free diet is eventually recommended, serologic testing is useful for monitoring adherence and response to the diet.
2
The tTG and EMA tests have excellent sensitivity and specificity. A recent screening study by Maki et al
19 of 3634 schoolchildren in Finland (7–16 years) found that each of these tests alone was approximately 95% sensitive. The combination of the tests resulted in a sensitivity and specificity of 100% and 99%, respectively.
19 Russo
20 found that the combination of IgA-AGA, IgG-AGA, and EMA tests resulted in 100% sensitivity and negative predictive values, with no false-negatives, in pediatric patients referred for duodenal biopsies (N=95).
Although it is not conclusive whether screening the general population for celiac disease should be undertaken, it is clear that physicians must consider testing in asymptomatic individuals who are at highest risk of developing the disease. This includes patients with first-degree relatives who were diagnosed with celiac disease. Some experts
2,21–25 also recommend that individuals with autoimmune disorders (eg, autoimmune liver disorders, type 1 diabetes mellitus), functional dyspepsia, idiopathic dilated cardiomyopathy, irritable bowel syndrome, unexplained fatigue, and certain neurologic disorders be evaluated for celiac disease.
Figure 5 illustrates the pretest and posttest probabilities that individuals with various associated medical conditions—as well as individuals in the general asymptomatic population—have celiac disease.
19,26 Clinicians should consider testing in any population whose pretest probability is elevated compared with the general asymptomatic population. When antibody tests are inconclusive, testing for the genetic markers HLA DQ2 and HLA DQ8 can accurately identify those at greatest risk.
3 It would be highly unlikely for an individual with a negative test result for these genetic markers to have celiac disease.
3
A diagnosis of celiac disease may seem obvious from results of serologic testing, but tissue biopsy remains the gold standard for diagnosis. In current practice, most biopsies are performed during upper endoscopy because this technique is more reliable than previous capsule-biopsy techniques.
2 Biopsy specimens should be obtained from the second or third section of the distal duodenum to avoid the architectural distortion produced by Brunner's glands (glandulae duodenales) or peptic duodenitis.
2
The classic lesion in untreated patients with celiac disease is characterized histologically by the absence of absorptive villi and by hyperplasia of crypts in the gastrointestinal tract.
2 These tissue changes are associated with an increased number of intraepithelial lymphocytes, as well as an increase in lymphocytes and plasma cells in the lamina propria.
2 The extent and severity of symptoms and malabsorption is directly influenced by the degree of enteropathy.
2
Celiac disease is more prevalent than previously believed, affecting approximately 1% of the US population. Patients who have this condition may experience a variety of symptoms, which are not limited to gastrointestinal problems.
Physicians need to be readily receptive to including celiac disease in their differential diagnoses of patients. Physicians should be especially sensitive to those patients who have the nonspecific complaints of arthralgia, myalgia, and fatigue, because celiac disease can easily remain undiagnosed in these individuals.
Diagnostic tests for celiac disease are readily available today—and less expensive than they were in the past. These tests have a high degree of sensitivity and specificity. Once celiac disease is diagnosed, a patient can benefit from a gluten-free diet, which promotes symptom relief and an improved quality of life. Proper diagnosis and treatment can help avoid the potentially serious long-term consequences of celiac disease.
Healthcare providers must become more familiar with celiac disease, a sometimes forgotten condition, to better serve their patients.
This study was funded in part by a contract from the Delaware Department of Health and Social Services, Division of Public Health.
The authors thank Cheryl Mongillo for manuscript preparation. The authors also gratefully acknowledge Robert Brodell, MD, for his generosity in providing
Figure 2. This study was funded in part by a contract from the Delaware Department of Health and Social Services, Division of Public Health.
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